Characterization of temperature-sensitive mutants of SV40.

  title={Characterization of temperature-sensitive mutants of SV40.},
  author={Akira Ishikawa and Tadashi Aizawa},
  journal={The Journal of general virology},
  volume={21 2},
We have isolated temperature-sensitive mutants of SV4 o in the lytic system by mutating the virus with nitrous acid or hydroxylamine, and characterized t I of them. Nine mutants were grouped into two complementation classes in the productive infection of CV-I cells. The first group contains a single mutant, which was defective in synthesizing virus DNA at the restrictive temperature, but induced the synthesis of cellular DNA. The mutants of the second class produced normal amounts of virus DNA… CONTINUE READING


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