Characterization of structural and functional domains of the anillin-related protein Mid1p that contribute to cytokinesis in fission yeast

@inproceedings{Saha2012CharacterizationOS,
  title={Characterization of structural and functional domains of the anillin-related protein Mid1p that contribute to cytokinesis in fission yeast},
  author={Shambaditya Saha and Thomas D. Pollard},
  booktitle={Molecular biology of the cell},
  year={2012}
}
Fission yeast cells depend on the anillin-related protein Mid1p for reliable cytokinesis. Insolubility limits the purification of full-length Mid1p for biophysical analysis, and lack of knowledge about the structural domains of Mid1p limits functional analysis. We addressed these limitations by identifying in a bacterial expression screen of random Mid1p fragments five soluble segments that can be purified and one insoluble segment. Using complementation experiments in Δmid1 cells, we tested… CONTINUE READING

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