Characterization of sterol-ester hydrolase in Saccharomyces cerevisiae.

  title={Characterization of sterol-ester hydrolase in Saccharomyces cerevisiae.},
  author={Shigeru Taketani and Takashi Osumi and H. Katsuki},
  journal={Biochimica et biophysica acta},
  volume={525 1},
A homgenate of Saccharomyces cerevisiae grown under semi-anaerobic as well as aerobic conditions was found to catalyze the hydrolysis of fatty acid esters of sterols in the presence of Triton X-100. The enzyme levels in cells grown under various conditions were similar and the enzyme had a broad substrate specificity for sterol esters. The enzyme was localized in the mitochondrial fraction for the aerobically grown cells and in the mitochondrial and cytosolic fractions for the semi… CONTINUE READING

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