Characterization of some major identity elements in plant alanine and phenylalanine transfer RNAs

Abstract

Alanine and phenylalanine tRNA sequences were amplified by PCR from Arabidopsis thaliana nuclear DNA using degenerate oligonucleotides which introduced specific mutations into the acceptor stem. The aminoacylation of T7 RNA polymerase transcripts of these sequences was investigated in vitro using partially purified bean alanyl- or phenylalanyl-tRNA… (More)
DOI: 10.1007/BF00019497

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