Characterization of soluble and membrane-bound family 3 lytic transglycosylases from Pseudomonas aeruginosa.

@article{Blackburn2002CharacterizationOS,
  title={Characterization of soluble and membrane-bound family 3 lytic transglycosylases from Pseudomonas aeruginosa.},
  author={Neil T. Blackburn and Anthony J Clarke},
  journal={Biochemistry},
  year={2002},
  volume={41 3},
  pages={1001-13}
}
Lytic transglycosylases cleave the beta,1-->4 glycosidic linkages between the N-acetylmuramoyl (MurNAc) and N-acetylglucosaminyl (GlcNAc) residues of peptidoglycan with the concomitant formation of 1,6-anhydro-N-acetylmuramyl reaction products. The genes encoding two hypothetical lytic transglycosylases were identified in the genome of Pseudomonas aeruginosa PAO1 by a BLAST search using membrane-bound lytic transglycosylase B (MltB) from Escherichia coli as the query. The two genes were… CONTINUE READING