Characterization of solubilized opioid receptors: reconstitution and uncoupling of guanine nucleotide-sensitive agonist binding.

@article{Ofri1992CharacterizationOS,
  title={Characterization of solubilized opioid receptors: reconstitution and uncoupling of guanine nucleotide-sensitive agonist binding.},
  author={Danielle Ofri and Ann M. Ritter and Younan Liu and Theresa Lee Gioannini and Jacob M. Hiller and Eric J. Simon},
  journal={Journal of neurochemistry},
  year={1992},
  volume={58 2},
  pages={628-35}
}
Opioid receptors were solubilized from bovine striatal membranes with the zwitterionic detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate-(CHAPS). High concentrations of NaCl (0.5-1.0 M) were necessary to ensure optimal yields, which ranged from 40 to 50% of membrane-bound receptors. This requirement was found to be specific for sodium, with only lithium able to substitute partially, as previously reported for solubilization with digitonin. Opioid antagonists, but not agonists… CONTINUE READING