Characterization of simple and complex hepatitis C virus quasispecies by heteroduplex gel shift analysis: correlation with nucleotide sequencing.

@article{Wilson1995CharacterizationOS,
  title={Characterization of simple and complex hepatitis C virus quasispecies by heteroduplex gel shift analysis: correlation with nucleotide sequencing.},
  author={J. John Wilson and Stephen J. Polyak and Tim D. Day and David R. Gretch},
  journal={The Journal of general virology},
  year={1995},
  volume={76 ( Pt 7)},
  pages={
          1763-71
        }
}
In infected humans, hepatitis C virus (HCV) exists as a quasispecies typically characterized by multiple nucleotide substitutions within the second envelope gene hypervariable region 1 (HVR1). In the current study, we used heteroduplex gel shift analysis (GSA) of HVR1 sequences amplified directly from patients' sera to define two patterns of HCV quasispecies: (i) simple quasispecies, which gave a mostly homogeneous gel shift profile with a single predominant band and (ii) complex quasispecies… 
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