Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris

@article{Fong2007CharacterizationOS,
  title={Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris},
  author={Mun Yik Fong and Yee Ling Lau and Mohamed Zulqarnain},
  journal={Biotechnology Letters},
  year={2007},
  volume={30},
  pages={611-618}
}
The surface antigen 2 (SAG2) gene of the protozoan parasite, Toxoplasma gondii, was cloned and extracellularly expressed in the yeast Pichia pastoris. The effectiveness of the secreted recombinant SAG2 (rSAG2-S) as a serodiagnosis reagent was assessed by western blots and ELISA. In the western blot assay, rSAG2-S reacted with all Toxoplasma-antibody positive human serum samples but not with Toxoplasma-negative samples. In the ELISA, rSAG2-S yielded sensitivity rates ranging from 80% (IgG… CONTINUE READING
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A (1998) The conformation

  • A Jacquet, C Dubeaux, Bollen
  • 1998

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