Characterization of rice α-amylase isozymes expressed by Saccharomyces cerevisiae

@article{Terashima1995CharacterizationOR,
  title={Characterization of rice α-amylase isozymes expressed by Saccharomyces cerevisiae},
  author={Masaaki Terashima and Shigeo Katoh and Bruce Robert Thomas and Raymond L. Rodriguez},
  journal={Applied Microbiology and Biotechnology},
  year={1995},
  volume={43},
  pages={1050-1055}
}
Two rice α-amylase isozymes, AmylA and Amy3D, were produced by secretion from genetically engineered strains of Saccharomyces cerevisiae. They have distinct differences in enzymatic characteristics that can be related to the physiology of the germinating rice seed. The rice isozymes were purified with immunoaffinity chromatography. The pH optima for amy3D (pH optimum 5.5) and Amy1A (pH optimum 4.2) correlate with the pH of the endosperm tissue at the times in rice seedling development when… CONTINUE READING

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