Characterization of recombinant Streptococcus mitis-derived human platelet aggregation factor.

@article{Ohkuni2012CharacterizationOR,
  title={Characterization of recombinant Streptococcus mitis-derived human platelet aggregation factor.},
  author={Hisashi Ohkuni and Hideaki Nagamune and Nana Ozaki and Atsushi Tabata and Yuko Todome and Yukino Watanabe and Hidemi Takahashi and Kazuto Ohkura and Hiroki Kourai and Hiroki Ohtsuka and Vincent A. Fischetti and John B. Zabriskie},
  journal={APMIS : acta pathologica, microbiologica, et immunologica Scandinavica},
  year={2012},
  volume={120 1},
  pages={56-71}
}
We previously purified Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) from the culture supernatant of S. mitis strain Nm-65, isolated from the tooth surface of a patient with Kawasaki disease. Here we produced recombinant Sm-hPAF protein (rSm-hPAF) in Escherichia coli, to determine whether rSm-hPAF conserves its platelet aggregation activity. rSm-hPAF precursor (665 amino acids) shows up to 36-56% identity with the family of cholesterol-dependent cytolysins (CDCs), and… CONTINUE READING