Characterization of raffinose synthase from rice (Oryza sativa L. var. Nipponbare)

  title={Characterization of raffinose synthase from rice (Oryza sativa L. var. Nipponbare)},
  author={Suhong Li and Tuoping Li and Wook-Dong Kim and Motomitsu Kitaoka and Shigeki Yoshida and Mitsutoshi Nakajima and Hideyuki Kobayashi},
  journal={Biotechnology Letters},
The putative raffinose synthase gene from rice was cloned and expressed in Escherichia coli. The enzyme displayed an optimum activity at 45°C and pH 7.0, and a sulfhydryl group was required for its activity. The enzyme was specific for galactinol and p-nitrophenyl-α-d-galactoside as galactosyl donors, and sucrose, lactose, 4−β-galactobiose, N-acetyl-d-lactosamine, trehalose and lacto-N-biose were recognized as galactosyl acceptors. 

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