Characterization of proteoglycan accumulation during formation of cartilagenous tissue in vitro.

@article{Boyle1995CharacterizationOP,
  title={Characterization of proteoglycan accumulation during formation of cartilagenous tissue in vitro.},
  author={Jeffery Boyle and Bing Luan and Tony F. Cruz and Rita A Kandel},
  journal={Osteoarthritis and cartilage},
  year={1995},
  volume={3 2},
  pages={
          117-25
        }
}
In order to study proteoglycan retention and accumulation, we optimized a chondrocyte cell culture system in which isolated bovine articular chondrocytes accumulate extracellular matrix to form a continuous layer of cartilagenous tissue. The tissue can attain a thickness of up to 110 microns by 35 days. The cells synthesize large keratan sulfate containing proteoglycans and type II collagen indicating that the chondrocytes maintain their phenotype in these culture conditions. Matrix… CONTINUE READING