Characterization of proteinase activation dynamics by capillary electrophoresis conjugating with fluorescent protein-based probe.

@article{Zhou2006CharacterizationOP,
  title={Characterization of proteinase activation dynamics by capillary electrophoresis conjugating with fluorescent protein-based probe.},
  author={Shixia Zhou and Juqiang Lin and Wei Du and Zhihong Zhang and Qingming Luo and Bi-Feng Liu and Yiqun Dai},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2006},
  volume={844 1},
  pages={158-62}
}
In this paper, a novel strategy was reported to characterize dynamics of proteinase activation based on capillary electrophoresis (CE), using caspase-2 as the model system. A fusion protein conjugating an amino acid sequence VDVAD with two fluorescent proteins enhanced cyan fluorescence protein (ECFP) and red fluorescence protein (DsRed), ECFP-VDVAD-DsRed, was specially designed and expressed in HeLa cells as the substrate of proteinase caspase-2. In this substrate, the sequence VDVAD could be… CONTINUE READING