Characterization of protein kinase C isoforms in primary cultured cerebellar granule cells

@article{Popp2006CharacterizationOP,
  title={Characterization of protein kinase C isoforms in primary cultured cerebellar granule cells},
  author={Robert L. Popp and Oscar Vel{\'a}squez and Jason Bland and Peter Hughes},
  journal={Brain Research},
  year={2006},
  volume={1083},
  pages={70-84}
}
Protein kinase C (PKC) is a family of serine/threonine kinases comprised of 10 isoforms. Although commercial antibodies are available for all 10 isoforms, the specificity of these antibodies has been questioned. We have identified immunoblot conditions in which commercially purchased PKC antibodies are specific for their respective isoform. We then used these conditions to determine that PKC isoforms alpha, betaI, betaII, delta, epsilon, gamma, lambda, theta, and zeta are present in rat primary… CONTINUE READING

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