Characterization of protein--DNA interactions using surface plasmon resonance spectroscopy with various assay schemes.
@article{Teh2007CharacterizationOP, title={Characterization of protein--DNA interactions using surface plasmon resonance spectroscopy with various assay schemes.}, author={Huey Fang Teh and Wendy Y X Peh and X. Su and J. Thomsen}, journal={Biochemistry}, year={2007}, volume={46 8}, pages={ 2127-35 } }
Specific protein-DNA interactions play a central role in transcription and other biological processes. A comprehensive characterization of protein-DNA interactions should include information about binding affinity, kinetics, sequence specificity, and binding stoichiometry. In this study, we have used surface plasmon resonance spectroscopy (SPR) to study the interactions between human estrogen receptors (ER, alpha and beta subtypes) and estrogen response elements (ERE), with four assay schemes… CONTINUE READING
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