Characterization of protective extracellular membrane-derived vesicles produced by Streptococcus pneumoniae.

@article{OlayaAbril2014CharacterizationOP,
  title={Characterization of protective extracellular membrane-derived vesicles produced by Streptococcus pneumoniae.},
  author={Alfonso Olaya-Abril and Rafael C Prados-Rosales and Michael James McConnell and Reyes Mart{\'i}n-Pe{\~n}a and Jos{\'e} A Gonz{\'a}lez-Reyes and Irene Jim{\'e}nez-Mungu{\'i}a and L{\'i}dia G{\'o}mez-Gasc{\'o}n and Javier Guti{\'e}rrez Fern{\'a}ndez and Jose L. Luque‐Garcia and Carlos Garc{\'i}a-Lid{\'o}n and H{\'e}ctor Est{\'e}vez and Jer{\'o}nimo Pach{\'o}n and Ignacio Obando and Arturo Casadevall and Liise-Anne Pirofski and Manuel J. Rodr{\'i}guez-Ortega},
  journal={Journal of proteomics},
  year={2014},
  volume={106},
  pages={
          46-60
        }
}
UNLABELLED Extracellular vesicles are produced by many pathogenic microorganisms and have varied functions that include secretion and release of microbial factors, which contribute to virulence. Very little is known about vesicle production by Gram-positive bacteria, as well as their biogenesis and release mechanisms. In this work, we demonstrate the active production of vesicles by Streptococcus pneumoniae from the plasma membrane, rather than being a product from cell lysis. We biochemically… Expand
Immunomodulatory Effects of Pneumococcal Extracellular Vesicles on Cellular and Humoral Host Defenses
TLDR
The data suggest that EVs may act in an immunomodulatory manner by allowing delivery of vesicle-associated proteins and other macromolecules into host cells and help pneumococci to avoid complement deposition and phagocytosis-mediated killing, thereby possibly contributing to the symptoms found in pneumococcal infections. Expand
Listeria monocytogenes virulence factors are secreted in biologically active Extracellular Vesicles
TLDR
It is demonstrated that L. monocytogenes utilize EVs for toxin release and implicate these structures in mammalian cytotoxicity and the deletion of plcA increased EV toxicity, suggesting PI-PLC can restrain LLO activity. Expand
Potential Usefulness of Streptococcus pneumoniae Extracellular Membrane Vesicles as Antibacterial Vaccines
TLDR
Although the S. pneumoniae bacteria were highly pathogenic in a mouse model, the EMVs purified from these bacteria showed low pathological activity both in cell culture and in mice, suggesting their potential as new vaccine candidates. Expand
Streptococcal Extracellular Membrane Vesicles Are Rapidly Internalized by Immune Cells and Alter Their Cytokine Release
TLDR
A better understanding is provided of gram-positive streptococcal MVs and their potential to elicit a protective immune response and they could offer an innovative avenue for safe and effective cell-free vaccination against pneumococcal infections. Expand
The Contribution of Membrane Vesicles to Bacterial Pathogenicity in Cystic Fibrosis Infections and Healthcare Associated Pneumonia
TLDR
The functional aspects of MVs secreted by bacteria associated with cystic fibrosis and nosocomial pneumonia are discussed, which mainly focus on how MVs are involved in virulence, antibiotic resistance, biofilm development and inflammation that consequently aid these bacterial infections. Expand
Release of Staphylococcus aureus extracellular vesicles and their application as a vaccine platform
TLDR
It is demonstrated that EVs purified from a S. aureus mutant that is genetically engineered to express detoxified cytolysins are immunogenic in mice, elicit cy tolysin-neutralizing antibodies, and protect the animals in a lethal sepsis model. Expand
A Two-Component Regulatory System Impacts Extracellular Membrane-Derived Vesicle Production in Group A Streptococcus
TLDR
It is shown that GAS naturally produces extracellular vesicles with a unique lipid composition that are laden with proteins and RNAs, and that genetic disruption of the virulence-associated two-component regulator CovRS leads to an increase in vesicle production. Expand
Pathogenesis Mediated by Bacterial Membrane Vesicles.
TLDR
An overview of the literature regarding bacterial membrane vesicles (BMVs) produced by pathogenic and commensal bacteria is provided and the mechanisms of BMV biogenesis and their roles in assisting bacterial survival are highlighted, in addition to discussing their functions in promoting disease pathologies and their potential use as novel therapeutic strategies. Expand
The biogenesis of extracellular vesicles from Staphylococcus aureus and their application as a novel vaccine platform
TLDR
Novel mechanisms underlying S. aureus EV production are revealed and the usefulness of EVs as a novel S.aureus vaccine platform is highlighted. Expand
Bacterial membrane vesicles: Biogenesis, immune regulation and pathogenesis
TLDR
This review will highlight findings identifying the contribution of extracellular vesicles produced by Gram‐positive bacteria, fungi, parasites, and infected host cells in mediating pathogenesis in addition to the functions of MVs produced by commensal bacteria. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 57 REFERENCES
Extracellular Vesicles Produced by Cryptococcus neoformans Contain Protein Components Associated with Virulence
TLDR
An efficient and general mechanism of secretion of pathogenesis-related molecules in C. neoformans is revealed, suggesting that extracellular vesicles function as “virulence bags” that deliver a concentrated payload of fungal products to host effector cells and tissues. Expand
Staphylococcus aureus Produces Membrane-Derived Vesicles That Induce Host Cell Death
TLDR
This study is the first report that S. aureus MVs are an important vehicle for delivery of bacterial effector molecules to host cells and subsequent cytotoxicity. Expand
Gram‐positive bacteria produce membrane vesicles: Proteomics‐based characterization of Staphylococcus aureus‐derived membrane vesicles
TLDR
It is found for the first time that Gram‐positive bacteria naturally produce MVs into the extracellular milieu and these observations suggest that the secretion of MVs is an evolutionally conserved, universal process that occurs from simple organisms to complex multicellular organisms. Expand
Bacillus anthracis produces membrane-derived vesicles containing biologically active toxins
TLDR
The results indicate that toxin secretion in B. anthracis is, at least, partially vesicle-associated, thus allowing concentrated delivery of toxin components to target host cells, a mechanism that may increase toxin potency. Expand
Staphylococcus aureus α-Toxin-Dependent Induction of Host Cell Death by Membrane-Derived Vesicles
TLDR
It is shown that cholesterol-dependent fusion of S. aureus MVs with the plasma membrane represents a route for delivery of a key virulence factor, α-toxin (α-hemolysin; Hla) to human cells, and is the first recognition of an S.aureus MV-associated factor contributing to host cell cytotoxicity. Expand
Streptococcus pneumoniae proteins released into medium upon inhibition of cell wall biosynthesis
TLDR
This process was studied in wild-type cells grown under lysis-permissive conditions as well as in an autolysin-deficient mutant, finding that Membrane vesicles could indeed be detected by electron microscopy of negative-stained secreted material. Expand
Mycobacteria release active membrane vesicles that modulate immune responses in a TLR2-dependent manner in mice.
TLDR
The results provide strong evidence that actively released mycobacterial vesicles are a delivery mechanism for immunologically active molecules that contribute to myc Cobacterial virulence. Expand
Vesicle and vesicle-free extracellular proteome of Paracoccidioides brasiliensis: comparative analysis with other pathogenic fungi.
TLDR
This unique proteomic analysis of extracellular vesicles and vesicle-free released proteins in a pathogenic fungus provides full comparison with other fungal extraceocytes and vESicle proteomes and broadens the current view on fungal secretomes. Expand
Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response
TLDR
This work provides physiological relevance for vesicle production as a protective mechanism, and demonstrates that production of bacterial outer membrane vesicles is a fully independent, general envelope stress response. Expand
Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans‐cell wall transfer of proteins and lipids in ascomycetes
TLDR
The results demonstrate that H. capsulatum can utilize a trans‐cell wall vesicular transport secretory mechanism to promote virulence and support the proposal that vesicle secretion is a general mechanism in fungi for the transport of macromolecules related to virulence. Expand
...
1
2
3
4
5
...