Characterization of porcine circovirus type 2 (PCV2) capsid particle assembly and its application to virus-like particle vaccine development

@article{Wu2012CharacterizationOP,
  title={Characterization of porcine circovirus type 2 (PCV2) capsid particle assembly and its application to virus-like particle vaccine development},
  author={Pei-Ching Wu and Wei-li Lin and Chi-Ming Wu and Jiun-Ni Chi and M N Chien and Chienjin Huang},
  journal={Applied Microbiology and Biotechnology},
  year={2012},
  volume={95},
  pages={1501-1507}
}
Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus-associated diseases in pigs. The sole structural capsid protein of PCV2, Cap, consists of major antigenic domains, but little is known about the assembly of capsid particles. The purpose of this study is to produce a large amount of Cap protein using Escherichia coli expression system for further studying the essential sequences contributing to formation of particles. By using codon optimization of rare… CONTINUE READING