Characterization of polymers of adenosine diphosphate ribose generated in vitro and in vivo.

@article{AlvarezGonzlez1987CharacterizationOP,
  title={Characterization of polymers of adenosine diphosphate ribose generated in vitro and in vivo.},
  author={Rafael Alvarez-Gonz{\'a}lez and Myron K. Jacobson},
  journal={Biochemistry},
  year={1987},
  volume={26 11},
  pages={3218-24}
}
Methods have been developed and applied to determine the size and branching frequency of polymers of ADP-ribose synthesized in nucleotide-permeable cultured mouse cells and in intact cultured cells. Polymers were purified by affinity chromatography with a boronate resin and were fractionated according to size molecular sieve high-performance liquid chromatography. Fractions were enzymatically digested to nucleotides, which were separated by strong anion exchange high-performance liquid… CONTINUE READING