Characterization of ploidy levels of wheat microspore-derived plants using laser flow cytometry

  title={Characterization of ploidy levels of wheat microspore-derived plants using laser flow cytometry},
  author={Kyung-Moon Kim and P. Stephen Baenziger and Jan J. Rybczyński and Kathiravetpillai Arumuganathan},
  journal={In Vitro Cellular & Developmental Biology - Plant},
The purpose of this study was to determine simply and accurately ploidy levels as estimated by changes in nuclear DNA content of wheat (Triticum aestivum L.) plants regenerated from microspore-derived embryos. Using flow cytometry, the nuclear DNA content of green (83) and albino (222) plants derived using anther culture of ‘Bobwhite’ and ‘Pavon 76’, and of their reciprocal F1 hydrids was estimated. The average DNA concent of the Bobwhite and Pavon 76 standards was 32.46 and 31.28 per nucleus… CONTINUE READING
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