Characterization of phosphoglycan-containing secretory products of Leishmania

@article{Ilg1994CharacterizationOP,
  title={Characterization of phosphoglycan-containing secretory products of Leishmania},
  author={Thomas Ilg and York Stierhof and Martin Wiese and Malcolm J. McConville and Peter Overath},
  journal={Parasitology},
  year={1994},
  volume={108},
  pages={S63 - S71}
}
SUMMARY This article presents an overview on phosphoglycan-containing components secreted by the insect and mammalian stages of several species of Leishmania, the causative agents of leishmaniasis in the Old and New World. Firstly, promastigotes of all three species considered, L. mexicana, L. donovani and L. major, shed lipophosphoglycan (LPG) into the culture medium possibly by release of micelles from the cell surface. Like the cell-associated LPG, culture supernatant LPG is arhphiphilic and… 

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TLDR
LPG is not essential for survival in the early blood-fed midgut but, along with other secreted phosphoglycan-containing glycoconjugates, can protect promastigotes from the digestive enzymes in the gut and, second, that LPG is required to mediate midGut attachment and to maintain infection in the fly during excretion of the digested blood meal.

Purification and Structural Characterization of a Filamentous, Mucin-like Proteophosphoglycan Secreted by Leishmania Parasites*

TLDR
The purification of this parasite antigen from Leishmania major culture supernatant is reported and its compositional, structural, and ultrastructural characterization as a highly unusual proteophosphoglycan (PPG) is reported.

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TLDR
It is proposed that monomeric or, possibly, oligomeric subunits synthesized in the cells are secreted into the flagellar pocket of Trypanosomatids, and polymer formation from subunits is suggested to occur in the lumen of the pocket before release into the culture medium or, naturally, into the gut of infected sandflies.

Characterization of a Novel GDP-mannose:Serine-protein Mannose-1-phosphotransferase from Leishmania mexicana *

TLDR
It is shown that Leishmania mexicana promastigotes contain a peptide-specific mannose-1-phosphotransferase (pep-MPT) activity that adds Manα1-P to serine residues in a range of defined peptides.

Stage-specific Proteophosphoglycan from Leishmania mexicana Amastigotes

TLDR
The variety of stage-specific glycan structures in Leishmania mexicana aPPG suggests the presence of developmentally regulated amastigote glycosyltransferases which may be potential anti-parasite drug targets.

Synthetic neoglycoconjugates of cell-surface phosphoglycans of Leishmania as potential anti-parasite carbohydrate vaccines

TLDR
Structural aspects of various phosphoglycans from Leishmania are discussed with the major emphasis on the chemical preparation of neoglycoconjugates (neoglycoproteins and neoglycolipids) based on Leishmanniasis lipophosphoglycan structures as well as the immunological evaluation for some of them as potential anti-leishmaniasis vaccines.

Biosynthesis of Leishmania lipophosphoglycan: solubilization and partial characterization of the initiating mannosylphosphoryltransferase.

TLDR
Results provide evidence that the initiating mannosylphosphoryltransferase is developmentally regulated during the life-cycle of the Leishmania parasite.
...

References

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TLDR
The mAbs are used to characterize changes in lipophosphoglycan structure, which occur in culture during the transition of promastigotes from the logarithmic to the stationary growth phase, and suggest the presence of capped phosphosaccharide repeats while the enzymically active glycoprotein subunit is modified by caps but probably not by repeats.

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The glycolipids of the protozoan Leishmania major strain LRC-L119 belong to a class of glycoinositol phospholipids (GIPL) that show partial structural homology to the phosphatidylinositol-containing

Characterization of polymer release from the flagellar pocket of Leishmania mexicana promastigotes

TLDR
It is proposed that monomeric or, possibly, oligomeric subunits synthesized in the cells are secreted into the flagellar pocket of Trypanosomatids, and polymer formation from subunits is suggested to occur in the lumen of the pocket before release into the culture medium or, naturally, into the gut of infected sandflies.

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TLDR
The enzyme purified from the culture medium of Leishmania mexicana is shown to be a complex composed of a predominant phosphorylated glycoprotein with a relative molecular mass of 100 kDa and noncovalently associated high molecular mass (proteo)phosphoglycans.

Monoclonal antibodies to Leishmania mexicana promastigote antigens. I. Secreted acid phosphatase and other proteins share epitopes with lipophosphoglycan.

TLDR
It is shown in this paper that monoclonal antibodies directed against LPG also react with an acid phosphatase secreted by L. mexicana promastigotes, and other parasite proteins appear to be modified by LPG epitopes.
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