Characterization of phloretin-sensitive urea export from the perfused rat liver.

@article{Dahl1996CharacterizationOP,
  title={Characterization of phloretin-sensitive urea export from the perfused rat liver.},
  author={Stephan vom Dahl and Dieter Haussinger},
  journal={Biological chemistry Hoppe-Seyler},
  year={1996},
  volume={377 1},
  pages={
          25-37
        }
}
In single pass perfused rat liver, rapid osmotic water shifts across the plasma membrane in response to hyperosmolar urea were followed by monitoring liver mass and transient concentrating or diluting effects on Na+ concentration in effluent perfusate. Sudden addition or removal of hyperosmolar urea (200mM, resulting in a step change of the perfusate osmolarity from 305 to 505 mosmol/l) had little effect on liver mass or Na+ activity in the effluent perfusate, suggesting that urea equilibrated… CONTINUE READING