Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.

@article{Tanaka1996CharacterizationOP,
  title={Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.},
  author={Hideo Tanaka and Ichio Shibagaki and Yutaka Shimada and Takashi Wagata and Masayuki Imamura and Kanji Ishizaki},
  journal={International journal of cancer},
  year={1996},
  volume={65 3},
  pages={372-6}
}
We screened 29 human esophageal squamous cell carcinoma (ESC) cell lines for mutations of the p53 gene through all coding exons and exon-intron junctions. Mutations were found in 22 cell lines (76%), consisting of 20 single-base substitutions, 2 small deletions and 1 single-base insertion. Out of 20 single-base substitution, 5 were located at the exon-intron junctions and mRNAs with abnormal splicing were detected by RT-PCR in 4 of them. A G:C to T:A transversion, which occurred rather… CONTINUE READING

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Researchers should have respect for the originator of the cell lines.

Clinical cancer research : an official journal of the American Association for Cancer Research • 2005

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