Characterization of oxytocin receptor expression and distribution in the pregnant sheep uterus.

@article{Wu1996CharacterizationOO,
  title={Characterization of oxytocin receptor expression and distribution in the pregnant sheep uterus.},
  author={W. X. Wu and J. G. Verbalis and G. E. Hoffman and Jan B. Derks and Peter W Nathanielsz},
  journal={Endocrinology},
  year={1996},
  volume={137 2},
  pages={
          722-8
        }
}
At the end of pregnancy, the myometrium becomes extremely sensitive to oxytocin (OT) as result of a dramatic increase in the number of OT receptors (OTR), indicating an important role for OTR in the process of labor. There are no studies in sheep in which the physical properties and histological distribution of OTR are evaluated in relation to parturition. Also, no studies have been performed in any species to simultaneously examine the distribution of OTR at the messenger RNA (mRNA) as well as… 
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54 Citations
Background Citations
21
Methods Citations
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Results Citations
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54 Citations

Citation Type

Citation Type

Uterine and placental expression of canine oxytocin receptor during pregnancy and normal and induced parturition.
TLDR
Findings suggest involvement of OTR in the signalling cascade leading to the prepartum release of prostaglandins from the pregnant canine uterus.
Investigation of the oxytocin receptor gene in sheep
TLDR
The aim of this study was to obtain and characterise OTR cDNA and OTR gene transcripts, to determine the O TR gene expression level in different tissue types, to investigate the role of exogenous steroids in the regulation of OTRGene expression, and to develop a gene expression system for OTR protein.
Oxytocin receptor gene expression in rat uterus: regulation by ovarian steroids.
TLDR
The results suggest that the decline of progesterone is necessary for the expression of the stimulatory effects of oestrogen on uterine OTR mRNA.
Oxytocin receptors in dioestrous and anoestrous canine uteri
TLDR
In conclusion, despite the apparent inactivity, the uterus of the non-pregnant bitch expresses OTR, and the distribution or relative expression of OTR does not differ between uterine horn and body in dioestrus or anoestrus except in LE.
Infusion of oxytocin induces successful delivery in prostanoid FP-receptor-deficient mice
Function and localization of oxytocin receptors in the reproductive tissue of rams.
TLDR
Findings indicate that oxytocin can modulate sperm transport in the ram testis and support a role for Oxytocin in promoting sperm transit is supported by the localization of oxytocIn receptors in the cauda epididymis and ductus deferens, and the presence of receptors on Leydig, Sertoli and epidIDymal epithelial cells provides further evidence that oxyoctocin may be involved in the local regulation of steroidogenesis.
Up-regulation of oxytocin receptor messenger ribonucleic acid and protein by estradiol in the cervix of ovariectomized rat.
TLDR
Estradiol-dependent activation of O TR gene expression and active OTR synthesis in smooth muscle cells account for the increased OTR level in rat cervix in vivo, in which progesterone acted as an antagonist of estradiol on OTR gene expression.
Characterization of decorin mRNA in pregnant intrauterine tissues of the ewe and regulation by steroids.
TLDR
It is observed that the fibroblasts are the predominant cell type in the pregnant sheep myometrium containing decorin mRNA, which suggests that increased decorin synthesis participates in the matrix changes that may play a role in myometrial activation.
Effect of labor induction on the expression of oxytocin receptor, cytochrome P450 aromatase, and estradiol receptor in the reproductive tract of the late-pregnant ewe.
TLDR
Induction of aromatase may facilitate the expression of endometrial estradiol and oxytocin receptors in the placentome and changes in endometrium rather than myometrial oxytoc in receptor may be important in inducing parturition.
Effect of pregnancy on endometrial expression of luteolytic pathway components in the mare.
TLDR
Gen and protein expression for PTGS2 and OXTR increased markedly between Days 14 and 21 of pregnancy suggesting that, although initial avoidance of luteolysis during pregnancy involves their suppression, this is a transient measure that delays rather than abolishes lutenolytic pathway generation.
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References

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TLDR
Results from investigations into the effects of estrogen and progesterone on the synthesis of OT by human chorio-decidua support the hypothesis of a paracrine network within human fetal membranes and decidua that may participate in regulating the timing of human birth.
Structure and expression of a human oxytocin receptor
TLDR
The structure and expression of the human oxytocin receptor complementary DNA isolated by expression cloning are reported and it is concluded that the increase in receptor number in the myometrium at labour is, at least in part, due to the increased in mRNA.
Changes in Oxytocin Receptor Messenger RNA in the Endometrium, Myometrium, Mesometrium, and Cervix of Sheep in Late Gestation and During Spontaneous and Cortisol-Induced Labor
TLDR
In the pregnant sheep, myometrial and endometrial oxytocin receptor mRNA increases significantly in both spontaneous and cortisol-induced labor as compared with appropriate controls, and there was no increase in oxytoc in receptor mRNA in either the cervix or the mesometrium.
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TLDR
It is proposed that the following sequence of events occurs in the initiation of labor in the rat: the decline in serum progesterone permits estradiol to stimulate the synthesis of estrogen receptors in the myometrium, which then triggers labor by interacting with circulating OT.
Changes in Oxytocin Receptor Messenger RNA in the Endometrium, Myometrium, Mesometrium, and Cervix of Sheep in Late Gestation and During Spontaneous and Cortisol-Induced Labor
  • W. Wu, P. Nathanielsz
  • Biology, Medicine
    The Journal of the Society for Gynecologic Investigation: JSGI
  • 1994
TLDR
In the pregnant sheep, myomttrial and endomTTrial oxytocin receptor mRNA increases significantly in both spontaneous and cortisol-induced labor as compared with appropriate controls, and there was no increase in oxytoc in receptor mRNA in either the cervix or the mesometrium.
Expression and localization of oxytocin receptor gene in human uterine endometrium in relation to the menstrual cycle.
TLDR
The sequential changes in oxytocin receptor mRNA expression in nonpregnant endometrium suggest some alternative biological function of oxytoc inendometrium during the menstrual cycle.
Oxytocin receptors and parturition. III. Increases in estrogen receptor and oxytocin receptor concentrations in the rat myometrium during prostaglandin F2 alpha-induced abortion.
TLDR
The administration of P to these rats inhibited the increases in concentration of receptors for E and OT on day 20 and prevented premature delivery, and PGF2α administration on day 15, when plasma P is near maximal, resulted in marginal changes in myometrial receptor concentrations and in uninterrupted pregnancy.
Rabbit uterine oxytocin receptors and in vitro contractile response: abrupt changes at term and the role of eicosanoids.
TLDR
A highly regulated physiological role for oxytocin in parturition which depends primarily on changes in receptor concentration is supported.
Characterization and localization of oxytocin receptors in the uterus and oviduct of the non-pregnant ewe using an iodinated receptor antagonist.
TLDR
Endometrial binding sites for [125I]OTA were consistently located on the luminal epithelium and epithelial cells lining secretory glands and competition studies with a number of oxytocin analogues and related peptides and the tissue distribution indicated that [125i]OTA bound to the ovine Oxytocin receptor.
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