Characterization of operating parameters for XMuLV inactivation by low pH treatment.

@article{Chinniah2016CharacterizationOO,
  title={Characterization of operating parameters for XMuLV inactivation by low pH treatment.},
  author={Shivanthi Chinniah and Peter Hinckley and Lisa Connell-Crowley},
  journal={Biotechnology progress},
  year={2016},
  volume={32 1},
  pages={
          89-97
        }
}
To ensure the viral safety of protein therapeutics made in mammalian cells, purification processes include dedicated viral clearance steps to remove or inactivate adventitious and endogenous viruses. One such dedicated step is low pH treatment, a robust and effective method commonly used in monoclonal antibody production to inactivate enveloped viruses. To characterize the operating space for low pH viral inactivation, we performed a statistically designed experiment evaluating the effect of pH… CONTINUE READING

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SHOWING 1-10 OF 33 REFERENCES

Standard Practice for Process for Inactivation of Rodent Retrovirus by pH

  • E ASTM.
  • West Chonshohocken, PA: ASTM International;
  • 2012
VIEW 6 EXCERPTS
HIGHLY INFLUENTIAL

Viral Clearance Using Traditional, Well-Understood Unit Operations (Session I): Low-pH Inactivation.

  • Qi Chen
  • Chemistry, Medicine
  • PDA journal of pharmaceutical science and technology
  • 2014
VIEW 6 EXCERPTS
HIGHLY INFLUENTIAL

Viral Inactivation: Low pH and Detergent.

VIEW 2 EXCERPTS

Protocol for Evaluation of Virus Inactivation Using Low-pH Treatment.

VIEW 1 EXCERPT