Characterization of novel acyl coenzyme A dehydrogenases involved in bacterial steroid degradation.

@article{Ruprecht2015CharacterizationON,
  title={Characterization of novel acyl coenzyme A dehydrogenases involved in bacterial steroid degradation.},
  author={Amanda Ruprecht and Jaymie Maddox and Alexander J Stirling and Nicole Visaggio and Stephen Y. K. Seah},
  journal={Journal of bacteriology},
  year={2015},
  volume={197 8},
  pages={1360-7}
}
UNLABELLED The acyl coenzyme A (acyl-CoA) dehydrogenases (ACADs) FadE34 and CasC, encoded by the cholesterol and cholate gene clusters of Mycobacterium tuberculosis and Rhodococcus jostii RHA1, respectively, were successfully purified. Both enzymes differ from previously characterized ACADs in that they contain two fused acyl-CoA dehydrogenase domains in a single polypeptide. Site-specific mutagenesis showed that only the C-terminal ACAD domain contains the catalytic glutamate base required for… CONTINUE READING

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