Characterization of non-toxic mutant toxins of Vero toxin 1 that were constructed by replacing amino acids in the A subunit.


Three mutant toxins of Vero toxin 1 (VT1) produced by Escherichia coli strains carrying mutant VT1 genes that were constructed by oligonucleotide-directed site-specific mutagenesis were purified to homogeneity. They are E167Q (glutamic acid at position 167 from the N-terminus of the A subunit was replaced by glutamine), R170L (arginine at position 170 from… (More)


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