Characterization of nicotine and cotinine N-glucuronidations in human liver microsomes.

@article{Nakajima2002CharacterizationON,
  title={Characterization of nicotine and cotinine N-glucuronidations in human liver microsomes.},
  author={Miki Nakajima and Eriko Tanaka and Jun-Tack Kwon and Tsuyoshi Yokoi},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2002},
  volume={30 12},
  pages={1484-90}
}
The nicotine and cotinine N-glucuronidations in human liver microsomes were characterized. The Eadie-Hofstee plots of nicotine N-glucuronidation in human liver microsomes were clearly biphasic, indicating the involvement of multiple enzymes. The apparent K(m) and V(max) values were 33.1 +/- 28.1 micro M and 60.0 +/- 21.0 pmol/min/mg and 284.7 +/- 122.0 micro M and 124.0 +/- 44.0 pmol/min/mg for the high- and low-affinity components, respectively, in human liver microsomes (n = 4). However, the… CONTINUE READING

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