Characterization of mycoplasma arginine deiminase expressed in E. coli and its inhibitory regulation of nitric oxide synthesis.

@article{Noh2002CharacterizationOM,
  title={Characterization of mycoplasma arginine deiminase expressed in E. coli and its inhibitory regulation of nitric oxide synthesis.},
  author={Eun Joo Noh and Sang Wook Kang and Yong Jae Shin and Dong Chung Kim and In Sun Park and Min Young Kim and Boe Gwun Chun and Bon Hong Min},
  journal={Molecules and cells},
  year={2002},
  volume={13 1},
  pages={137-43}
}
We previously reported that a cytostatic protein that is found in ASC-17D Sertoli cell-conditioned media was Mycoplasma arginine deiminase (ADI), which hydrolyzes L-arginine into L-citrulline and ammonia. Here, we report the over-expression of recombinant ADI (rADI) in E. coli and the down-regulation of lipopolysaccharide (LPS) induced-nitric oxide (NO) production by rADI treatment. We cloned the ADI gene from Mycoplasma arginini genomic DNA by a polymerase chain reaction, and changed five TGA… CONTINUE READING

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