Characterization of mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in an industrial Corynebacterium glutamicum strain.

@article{Ohnishi2008CharacterizationOM,
  title={Characterization of mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in an industrial Corynebacterium glutamicum strain.},
  author={Junko Ohnishi and Hiroshi Mizoguchi and Seiki Takeno and Masato Ikeda},
  journal={Mutation research},
  year={2008},
  volume={649 1-2},
  pages={239-44}
}
Mutations induced by classical whole-cell mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine (NTG) were determined for all genes of pathways from glucose to L-lysine in an industrial L-lysine producer of Corynebacterium glutamicum. A total of 50 mutations with a genome-wide distribution were identified and characterized for mutational types and mutagenic specificities. Those mutations were all point mutations with single-base substitutions and no deletions, frame shifts, and insertions were… CONTINUE READING