Characterization of mutations in the PAS domain of the EvgS sensor kinase selected by laboratory evolution for acid resistance in Escherichia coli

@inproceedings{Johnson2014CharacterizationOM,
  title={Characterization of mutations in the PAS domain of the EvgS sensor kinase selected by laboratory evolution for acid resistance in Escherichia coli},
  author={Matthew D. Johnson and James Bell and Kim Clarke and Rachel Chandler and Prachi Pathak and Yandong Xia and Robert L. Marshall and George Weinstock and Nicholas J. Loman and Peter J. Winn and Peter A. Lund},
  booktitle={Molecular microbiology},
  year={2014}
}
Laboratory-based evolution and whole-genome sequencing can link genotype and phenotype. We used evolution of acid resistance in exponential phase Escherichia coli to study resistance to a lethal stress. Iterative selection at pH 2.5 generated five populations that were resistant to low pH in early exponential phase. Genome sequencing revealed multiple mutations, but the only gene mutated in all strains was evgS, part of a two-component system that has already been implicated in acid resistance… CONTINUE READING
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