Characterization of mutants of Caulobacter crescentus defective in surface attachment of the paracrystalline surface layer.

@article{Walker1994CharacterizationOM,
  title={Characterization of mutants of Caulobacter crescentus defective in surface attachment of the paracrystalline surface layer.},
  author={Stephen G Walker and D. Nedra Karunaratne and N. O. M. Ravenscroft and John T. Smit},
  journal={Journal of bacteriology},
  year={1994},
  volume={176 20},
  pages={
          6312-23
        }
}
Strains of Caulobacter crescentus express a paracrystalline surface layer (S-layer) consisting of the protein RsaA. Mutants of C. crescentus NA1000 and CB2, isolated for their ability to grow in the absence of calcium ions, uniformly no longer had the S-layer attached to the cell surface. However, RsaA was still produced, and when colonies grown on calcium-sufficient medium were examined, large two-dimensional arrays of S-layer were found intermixed with the cells. Such arrays were not found in… CONTINUE READING

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