Characterization of monoclonal antibody to DNA.RNA and its application to immunodetection of hybrids.

@article{Boguslawski1986CharacterizationOM,
  title={Characterization of monoclonal antibody to DNA.RNA and its application to immunodetection of hybrids.},
  author={S J Boguslawski and Dr. Fannie E. Smith and Marek Michalak and K E Mickelson and C O Yehle and Walt Patterson and Ruth Carrico},
  journal={Journal of immunological methods},
  year={1986},
  volume={89 1},
  pages={123-30}
}
Monoclonal antibodies were raised to a DNA.RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase. A monoclonal antibody with the highest affinity and specificity was selected. This antibody bound the DNA.RNA heteropolymer and poly(I).poly(dC) equally but 100-fold higher levels of poly(A).poly(dT) were required to achieve a similar degree of binding in competitive binding assays using DNA.[3H]RNA. Single-stranded DNA, double-stranded… CONTINUE READING

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