Characterization of monoclonal antibodies to Marburg virus nucleoprotein (NP) that can be used for NP‐capture enzyme‐linked immunosorbent assay

  title={Characterization of monoclonal antibodies to Marburg virus nucleoprotein (NP) that can be used for NP‐capture enzyme‐linked immunosorbent assay},
  author={Masayuki Saijo and Masahiro Niikura and Akihiko Maeda and Tetsutaro Sata and Takeshi Kurata and Ichiro Kurane and Shigeru Morikawa},
  journal={Journal of Medical Virology},
After the first documented outbreak of Marburg hemorrhagic fever identified in Europe in 1967, several sporadic cases and an outbreak of Marburg hemorrhagic fever have been reported in Africa. In order to establish a diagnostic system for Marburg hemorrhagic fever by the detection of Marburg virus nucleoprotein, monoclonal antibodies to the recombinant nucleoprotein were produced. Two clones of monoclonal antibodies, MAb2A7 and MAb2H6, were efficacious in the antigen‐capture enzyme‐linked… 
Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic Marburgvirus.
Results suggest that the Ag-capture ELISA using the monoclonal antibodies, 2A7 and 2H6, is applicable to the diagnosis of MHF.
Production and characterization of monoclonal antibodies to nucleoprotein of Marburg virus.
A recombinant MARV-NP was successfully expressed by an Escherichia coli expression system and the isotypes of the three MAbs were tested to be IgG1(kappa) and all the MAbs recognized the same antigenic epitope.
Development of Recombinant Nucleoprotein-Based Diagnostic Systems for Lassa Fever
These LASV rNP-based diagnostics were confirmed to be useful in the diagnosis of LF even in institutes without a high containment laboratory, since the antigens can be prepared without manipulation of the infectious viruses.
Severe Fever with Thrombocytopenia Syndrome Virus Antigen Detection Using Monoclonal Antibodies to the Nucleocapsid Protein
The newly developed Ag-capture ELISA is useful for SFTS diagnosis in acute phase patients with high levels of viremia, particularly in patients suspected of having SFTS in Japan.
Ebolavirus Nucleoprotein C-Termini Potently Attract Single Domain Antibodies Enabling Monoclonal Affinity Reagent Sandwich Assay (MARSA) Formulation
The C-terminal domain of Ebolavirus NP is a strong attractant for antibodies and enables sensitive sandwich immunoassays to be rapidly generated using a single antibody clone, making this domain an ideal handle for monoclonal affinity reagent driven antigen sandwich assays for the Ebolvirus genus.
Unveiling a Drift Resistant Cryptotope within Marburgvirus Nucleoprotein Recognized by Llama Single-Domain Antibodies
It is shown that all sdAb bound the C-terminal region of NP, which was produced recombinantly to derive X-ray crystal structures of the three best performing antibody-antigen complexes, suggesting the antibodies acted as crystallization chaperones.
Monoclonal antibody to dengue capsid protein
It is suggested that detection of dengue capsid protein could be useful in the diagnosis of early d Dengue infection.
Recombinant Protein-Based Diagnostics for Viral Hemorrhagic Fevers
To get around the need for BSL-4 laboratories, diagnostic methods were developed; these methods use recombinant viral antigens of hemorrhagic fever viruses in which recombinant proteins of these viruses were expressed, and serological diagnostic methods have been developed using the recombinant genomes.
Laboratory detection and diagnosis of filoviruses
Recent progresses in the development of detection and diagnosis methods for EBOV and MARV are summarized.
Laboratory Diagnostic Systems for Ebola and Marburg Hemorrhagic Fevers Developed with Recombinant Proteins
Ebola virus and Marburg virus (EBOV and MARV, respectively) of the family Filoviridae cause hemorrhagic fever with high mortality rates, sometimes reaching 50 to 90% of infected individuals, in


Detection of Ebola Viral Antigen by Enzyme-Linked Immunosorbent Assay Using a Novel Monoclonal Antibody to Nucleoprotein
An ELISA system using a novel monoclonal antibody to the nucleoprotein (NP) detected the NP in subtype Reston-infected monkey specimens, while the background level in noninfected specimens was very low, suggesting the usefulness of the ELISA for laboratory diagnosis with clinical specimens.
Enzyme-Linked Immunosorbent Assays for Detection of Antibodies to Ebola and Marburg Viruses Using Recombinant Nucleoproteins
An immunoglobulin G (IgG) ELISA is developed using His-EBO-NP and the C-terminal halves of the NPs of EBO and MBG as antigens and shows high sensitivity and specificity in detecting EBO or MBG antibodies.
Antigen Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Reston Ebola Virus Nucleoprotein
This study developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res 2-1D8, specific to the NP of EBO-R, which are useful for the rapid detection of the NP in E BO-R-infected cynomolgus macaques.
Detection and molecular characterization of Ebola viruses causing disease in human and nonhuman primates.
The nonstructural secreted GP (SGP), the primary product of the GP gene, was more highly conserved than the structural GP, indicating different functional roles or evolutionary constraints for these proteins.
Clinical virology of Ebola hemorrhagic fever (EHF): virus, virus antigen, and IgG and IgM antibody findings among EHF patients in Kikwit, Democratic Republic of the Congo, 1995.
Ebola hemorrhagic fever (EHF) patients treated at Kikwit General Hospital during the 1995 outbreak were tested for viral antigen, IgG and IgM antibody, and infectious virus. Viral antigen could be
Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR
The suitability of the assays was exemplified by detection and quantification of viral RNA in serum samples of VHF patients, and all assays were thoroughly optimized and validated in terms of analytical sensitivity by using in vitro-transcribed RNA.
Risk Factors for Marburg Hemorrhagic Fever, Democratic Republic of the Congo
Primary transmission of Marburg virus to humans likely occurred via exposure to a still unidentified reservoir in the local mines, and secondary transmission appears to be less common withMarburg virus than with Ebola virus, the other known filovirus.
Epidemiologic investigation of Marburg virus disease, Southern Africa, 1975.
It is believed that during this outbreak the first Marburg virus infection occurred by vector-borne transmission from an arthropod yet to be identified, and that patients 2 and 3 acquired the disease by exposure to the oropharyngeal secretions of patients 1 and 2, respectively.
An introduction to Ebola: the virus and the disease.
A number of colleagues, both in the laboratory and in the field, agreed to prepare reports reflecting recent research, thus permitting this supplement to the Journal of Infectious Dis- Ebola, the
Outbreake of Marburg virus disease in Johannesburg.
The first recognised outbreak of Marburg virus disease in Africa, and the first since the original epidemic in West Germany and Yugoslavia in 1967, occurred in South Africa in February 1975. The