Characterization of monoclonal antibodies to an antigenic protein from Stachybotrys chartarum and its measurement in house dust.

@article{Xu2008CharacterizationOM,
  title={Characterization of monoclonal antibodies to an antigenic protein from Stachybotrys chartarum and its measurement in house dust.},
  author={Jianping Xu and Yinan Liang and Donald Belisle and J David Miller},
  journal={Journal of immunological methods},
  year={2008},
  volume={332 1-2},
  pages={121-8}
}
Using sera from atopic patients we have isolated an extracellular protein, which is antigenic in humans, from Stachybotrys chartarum sesu lato. Here we report the production of monoclonal antibodies to the protein and the development of a sensitive and specific assay to the target protein as well as analyses in house dust samples spiked with spores. The detection limit for the target antigen in house dust was approximately 0.2 ng/g dry weight house dust. This detection limit is comparable to… CONTINUE READING

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