Characterization of mitogen-stimulated porcine lymphocytes using a stable fluorescent dye (PKH2) and multicolor flow cytometry.

@article{Dorn2002CharacterizationOM,
  title={Characterization of mitogen-stimulated porcine lymphocytes using a stable fluorescent dye (PKH2) and multicolor flow cytometry.},
  author={Amelie Dorn and W Ray Waters and V M Byers and Bruce A Pesch and Michael J. Wannemuehler},
  journal={Veterinary immunology and immunopathology},
  year={2002},
  volume={87 1-2},
  pages={1-10}
}
Stimulation of lymphocyte proliferation using mitogens or specific antigens is a method that is used frequently to assess immune responsiveness. While useful, lymphocyte blastogenesis, or [3H]-thymidine incorporation, provides little information regarding the response of specific subsets to the stimulant. Here, we report that the fluorescent cell membrane probe, PKH2, is a useful tool for measuring the proliferation of porcine lymphocyte subpopulations by utilizing multicolor flow cytometry… CONTINUE READING

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