Characterization of microglia induced from mouse embryonic stem cells and their migration into the brain parenchyma

@article{Tsuchiya2005CharacterizationOM,
  title={Characterization of microglia induced from mouse embryonic stem cells and their migration into the brain parenchyma},
  author={Takahiro Tsuchiya and Kae Chang Park and Shinichi Toyonaga and Shoko Merrit Yamada and Keiji Shimizu},
  journal={Journal of Neuroimmunology},
  year={2005},
  volume={160},
  pages={210-218}
}
We derived microglia from mouse embryonic stem cells (ES cells) at very high density. Using the markers Mac1(+)/CD45(low) and Mac1(+)/CD45(high) to define microglia and macrophages, respectively, we show that Mac1(+) cells are induced by GM-CSF stimulation following neuronal differentiation of mouse ES cells using a five-step method. CD45(low) expression was high and CD45(high) expression was low on induced cells. We used a density gradient method to obtain a large amount of microglia-like… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 19 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 71 references

Macrophages and inflammation in the central nervous system

Trends in Neurosciences • 1993
View 6 Excerpts
Highly Influenced

Cytokines, chemokines, and cytokine receptors in human microglia.

Journal of neuroscience research • 2002
View 6 Excerpts
Highly Influenced

Immune function of microglia.

Glia • 2001
View 12 Excerpts
Highly Influenced

Microglia and macrophages are the major source of tumor necrosis factor in permanent middle cerebral artery occlusion in mice.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism • 2000
View 6 Excerpts
Highly Influenced

Murine microglial cells produce and respond to interleukin-18.

Journal of neurochemistry • 1999
View 8 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…