Characterization of metal chelate methacrylate monolithic disk for purification of polyclonal and monoclonal immunoglobulin G.

@article{Prasanna2010CharacterizationOM,
  title={Characterization of metal chelate methacrylate monolithic disk for purification of polyclonal and monoclonal immunoglobulin G.},
  author={Rajasekar R. Prasanna and Mookambeswaran Arunachalam Vijayalakshmi},
  journal={Journal of chromatography. A},
  year={2010},
  volume={1217 23},
  pages={3660-7}
}
Dynamic binding capacity (DBC) of commercial metal-chelate methacrylate monolith-convective interaction media (CIM) was performed with commercial human immunoglobulin G (IgG) (Cohn fraction II, III). Monoliths are an attractive stationary phase for purification of large biomolecules because they exhibit very low back pressure even at high flow rates and flow-unaffected binding properties. Adsorption of IgG onto CIM-IDA disk immobilized with Cu(2+), Ni(2+) and Zn(2+) were studied with Tris… CONTINUE READING