Characterization of membrane-bound and soluble catechol-O-methyltransferase from human frontal cortex.

@article{Jeffery1984CharacterizationOM,
  title={Characterization of membrane-bound and soluble catechol-O-methyltransferase from human frontal cortex.},
  author={D. Ross Jeffery and Jerome A. Roth},
  journal={Journal of neurochemistry},
  year={1984},
  volume={42 3},
  pages={826-32}
}
Catechol-O-methyltransferase (COMT; E.C. 2.1.1.6) from human frontal cortex occurs in both a soluble and membrane-bound form. Attempts to solubilize the membrane-bound transferase by repeated washing or by extraction into solutions of high ionic strength were unsuccessful. The finding that Triton X-100 was capable of solubilizing membrane-bound COMT… CONTINUE READING