Characterization of measles virus recovered from persistently infected Vero cells.

@article{Prasad1980CharacterizationOM,
  title={Characterization of measles virus recovered from persistently infected Vero cells.},
  author={I. Prasad and A. Mnere},
  journal={Japanese journal of medical science \& biology},
  year={1980},
  volume={33 6},
  pages={
          311-9
        }
}
  • I. Prasad, A. Mnere
  • Published 1980
  • Biology, Medicine
  • Japanese journal of medical science & biology
Vero cells were persistently infected with measles virus in medium with antimeasles serum. When antiserum was not added to the medium, the persistently infected cells (VMAS cells) proliferated in a cyclical manner alternating between extensive cell destruction and renewed cell growth. When these cells were grown at 33 C and 40 C, they released temperature-sensitive and temperature-dependent viruses. However, these viruses, upon further propagation, reverted to the wild type suggesting that such… Expand
2 Citations
Selection of temperature-sensitive mutants in persistent infection by parainfluenza virus type 3.
  • D. Hodes
  • Biology, Medicine
  • The Journal of general virology
  • 1982
TLDR
Vero cells persistently infected with parainfluenza virus type 3 (para 3) were examined for the production of temperature-sensitive mutants, and these mutants proved to be unstable when removed from the milieu of persistent infection. Expand
Properties of temperature-sensitive mutants of parainfluenza virus type 3 selected during the course of persistent infection
TLDR
Three mutants that were selected during the course of persistent infection of Vero cells by parainfluenza virus type 3 demonstrated leakiness when infecting cells at high MOI and interfered with the growth of wild type virus, apparently by inhibiting a step prior to RNA synthesis. Expand

References

SHOWING 1-10 OF 12 REFERENCES
Isolation and preliminary characterization of temperature-sensitive mutants of measles virus
TLDR
Twenty-four genetically stable temperature-sensitive mutants of measles virus isolated after mutangenesis by 5-azacytidine, 5 fluorouracil, or proflavine were found defective in both hemolysin antigen synthesis and cell fusion "from within," supporting the unitary hypothesis for these functions. Expand
Fluctuations and distribution of measles virus antigens in chronically infected cells
TLDR
The quantitative fluctuations of measles virus antigens at the cell surface in chronically infected cell cultures measured by haemadsorption and indirect membrane immunofluorescence are reported. Expand
Development of measles virus in vitro.
TLDR
The sequence of development of measles virus in cells cultivated in vitro was studied by electron microscopic techniques and found cytoplasmic tubule formation was associated with active budding and high yields of virus, whereas when intranuclear formation was predominant, little or no budding was detected and viral yields were low. Expand
Viral RNA synthesis in measles virus-infected cells.
TLDR
Sedimentation analysis of measles virus-specific cytoplasmic RNA at different times after infection revealed several size classes, with a temperature-sensitive mutant synthesis of all three major components turned off simultaneously by transfer of infected cultures from permissive to nonpermissive temperature. Expand
Temperature-Sensitive Defect of Mutants Isolated from L Cells Persistently Infected with Newcastle Disease Virus
TLDR
The temperature-sensitive defects of virus mutants isolated from L cells persistently infected with Newcastle disease virus (NDV) suggested that there may be two types of ts defects in NDV(pi), both associated with virus-specific RNA polymerase activity. Expand
State of the viral DNA in rat cells transformed by polyoma virus. I. Virus rescue and the presence of nonintergrated viral DNA molecules
TLDR
The interaction of polyoma virus with a continuous line of rat cells was studied, finding that these cells did not produce infectious virus, but in all clones tested virus was rescuable upon fusion with permissive mouse cells. Expand
Antibody-induced redistribution of measles virus antigens on the cell surface.
TLDR
Capping was inhibited with oligomycin D, vinblastine sulfate, colchicine, and cytochalasin B, suggesting that ATP production and functioning microfilaments and microtubles are involved in the capping process. Expand
The effects of actinomycin D on RNA synthesis in measles virus-infected cells.
The effect of actinomycin D on the production of genomic RNA, messenger RNA and protein in measles virus-infected cells were investigated. It was found that whereas high concentrations of the drugExpand
Biological and biochemical characterization of a latent subacute sclerosing panencephalitis (SSPE) virus infection in tissue culture
The present investigation describes the biological and biochemical properties of a persistent SSPE virus infection. Persistently infected cells were derived by cocultivation of infected brain cellsExpand
The effects of actinomycin D on RNA synthesis
  • 1977
...
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