Characterization of manganese(II) binding site mutants of manganese peroxidase.

  title={Characterization of manganese(II) binding site mutants of manganese peroxidase.},
  author={Kuki Kishi and M A Kusters-van Someren and Mary B. Mayfield and J Sun and Thomas M. Loehr and Michael H. Gold},
  volume={35 27},
A series of site-directed mutants, E35Q, E39Q, and E35Q-D179N, in the gene encoding manganese peroxidase isozyme 1 (mnp1) from Phanerochaete chrysosporium, was created by overlap extension, using the polymerase chain reaction. The mutant genes were expressed in P. chrysosporium during primary metabolic growth under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter. The mutant manganese peroxidases (MnPs) were purified and characterized. The molecular masses of the mutant… CONTINUE READING