Characterization of mammalian RAD51 double strand break repair using non-lethal dominant-negative forms.

@article{Lambert2000CharacterizationOM,
  title={Characterization of mammalian RAD51 double strand break repair using non-lethal dominant-negative forms.},
  author={Sarah R. Lambert and Bernard S Lopez},
  journal={The EMBO journal},
  year={2000},
  volume={19 12},
  pages={3090-9}
}
In contrast to yeast RAD51, mammalian mRAD51 is an essential gene. Its role in double strand break (DSB) repair and its consequences on cell viability remain to be characterized precisely. Here, we used a hamster cell line carrying tandem repeat sequences with an I-SCE:I cleavage site. We characterized conservative recombination after I-SCE:I cleavage as gene conversion or intrachromatid crossing over associated with random reintegration of the excised reciprocal product. We identified two… CONTINUE READING