Characterization of malignant rat keratinocytes in culture following the induction of oral squamous cell carcinomas in vivo.

@article{Crane1986CharacterizationOM,
  title={Characterization of malignant rat keratinocytes in culture following the induction of oral squamous cell carcinomas in vivo.},
  author={Isabel Joan Crane and Jakob Luker and Andrea Stone and Crispian Scully and S. S. Prime},
  journal={Carcinogenesis},
  year={1986},
  volume={7 10},
  pages={1723-7}
}
An in vivo model of oral epithelial carcinogenesis in rats has been established successfully in cell culture. Oral carcinomas of the tongue and palate were induced in Sprague-Dawley male rats by painting their palates three times weekly for 7-8 months with 0.5% (w/v) 4-nitroquinoline-N-oxide. Oral keratinocytes from malignant and untreated control tissues were cultivated using 3T3 fibroblast support. Both the normal and malignant cells stained positively with an anti-human keratin polyclonal… CONTINUE READING