Characterization of long term hormonal cell cultures from the MtT/w15 prolactin- and growth hormone-secreting rat pituitary tumor.

Abstract

PRL-secreting epithelial cell lines (PECL) derived from Furth's MtT/W15 transplantable rat pituitary tumor have been studied as monolayer cultures for up to 18 months. Tumor epithelial cells were mechanically dispersed, plated at high density, and selectively enriched by picking and pooling colonies, thereby avoiding single cell-cloning conditions, in order… (More)

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