Characterization of lipid-laden aortic cells from cholesterol-fed rabbits. I. Resolution of aortic cell populations by metrizamide density gradient centrifugation.


Enzymatically isolated aortic cells from control and cholesterol-fed rabbits were subjected to isopycnic density gradient centrifugation in Metrizamide. Control cells formed a single band equilibrating at a median density of 1.13 to 1.14, while cells isolated from atheromatous aortas formed two distinct bands, one equilibrating in the density range of 1.12… (More)