Characterization of leptospira isolates from animals and humans: phylogenetic analysis identifies the prevalence of intermediate species in India

@inproceedings{Balamurugan2013CharacterizationOL,
  title={Characterization of leptospira isolates from animals and humans: phylogenetic analysis identifies the prevalence of intermediate species in India},
  author={Vinayagamurthy Balamurugan and Nidaghatta L Gangadhar and Nagalingam Mohandoss and Sushma Rahim Assadi Thirumalesh and Moushumi Dhar and Rajeswari Shome and Paramanandham Krishnamoorthy and Krishnamsetty Prabhudas and H. Sheik Mujibur Rahman},
  booktitle={SpringerPlus},
  year={2013}
}
In this study, 191 culture isolates were recovered from suspected samples of animals and humans in Ellinghausen McCullough Johnson and Harris (EMJH) medium and assessed for its morphological features by dark field microscopy. Extracted DNA from individual culture was subjected to different PCR assays for identification and characterization of leptospira. Out of 99 positive leptospira cultures, 52 pathogenic leptospira isolates were characterized at species level by using partial RNA polymerase… CONTINUE READING