Characterization of iron (II).bleomycin-mediated RNA strand scission.

  title={Characterization of iron (II).bleomycin-mediated RNA strand scission.},
  author={Charles E. Holmes and Barrie J. Carter and Sidney M Hecht},
  volume={32 16},
The ability of iron(II).bleomycin to mediate RNA degradation was further characterized. At micromolar concentrations, FeII.BLM was shown to effect cleavage of Escherichia coli tRNA(1His) and a Schizosaccharomyces pombe amber suppressor tRNA construct in an efficient fashion. In contrast, E. coli tRNA(Cys) and yeast mitochondrial tRNA(Asp) and tRNA(fMet) precursors were not substrates for FeII.BLM. Also shown to be a good substrate for cleavage by FeII.BLM was yeast 5S ribosomal RNA. Since HIV-1… CONTINUE READING