Characterization of inside-out oriented H+-ATPases in cholate-pretreated renal brush-border membrane vesicles

@article{Simon1990CharacterizationOI,
  title={Characterization of inside-out oriented H+-ATPases in cholate-pretreated renal brush-border membrane vesicles},
  author={Bernd Simon and Gerhard Burckhardt},
  journal={The Journal of Membrane Biology},
  year={1990},
  volume={117},
  pages={141-151}
}
Exposure of porcine renal brush-border membrane vesicles to 1.2% cholate and subsequent detergent removal by dialysis reorients almost all N-ethylmaleimide (NEM)-sensitive ATPases from the vesicle inside to the outside. ATP addition to cholate-pretreated, but not to intact, vesicles causes H+ uptake as visualized by the ΔpH indicator, acridine organge. The reoriented H+-pump is electrogenic because permeant extravesicular anions or intravesicular K+ plus valinomycin enhance H+ transport. ATP… CONTINUE READING
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ATP-driven H + transport in intact and reconstituted rat renal brush-border membrane vesicles

  • G. Burckhardt, F. Turrini, B. Simon
  • Kidney Int
  • 1988
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