Characterization of immobilized nuclease P1.

@article{Ying2007CharacterizationOI,
  title={Characterization of immobilized nuclease P1.},
  author={Quo-Qing Ying and Lu-E Shi and Xiaoying Zhang and Wei Chen and Yu Peng Yi},
  journal={Applied biochemistry and biotechnology},
  year={2007},
  volume={136 1},
  pages={
          119-26
        }
}
To improve the efficiency of the use of nuclease P1, enzyme immobilization technology was applied using nuclease P1. Characterization of immobilized nuclease P1 on different supports was studied. The results showed that the optimum pH and temperature of nuclease P1 immobilized via different supports were enhanced. The immobilized enzyme was obviously stable when stored for long periods and was reusable. The best results were obtained when nuclease P1 was immobilized on chitosan nanoparticles… CONTINUE READING

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