Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus.

@article{Bryant1989CharacterizationOH,
  title={Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus.},
  author={Frank O. Bryant and Michael W. W. Adams},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 9},
  pages={5070-9}
}
The archaebacterium, Pyrococcus furiosus, grows optimally at 100 degrees C by a fermentative type metabolism in which H2 and CO2 are the only detectable products. The organism also reduces elemental sulfur (S0) to H2S. Cells grown in the absence of S0 contain a single hydrogenase, located in the cytoplasm, which has been purified 350-fold to apparent homogeneity. The yield of H2 evolution activity from reduced methyl viologen at 80 degrees C was 40%. The hydrogenase has a Mr value of 185,000… CONTINUE READING