Characterization of human thyroxine-binding globulin. Evidence for a single polypeptide chain.

@article{Gershengorn1977CharacterizationOH,
  title={Characterization of human thyroxine-binding globulin. Evidence for a single polypeptide chain.},
  author={Marvin C Gershengorn and S y Cheng and R. E. Lippoldt and Reginald S. A. Lord and Jeffrey Robbins},
  journal={The Journal of biological chemistry},
  year={1977},
  volume={252 23},
  pages={8713-8}
}
Thyroxine-binding globulin (TBG) was purified from fresh human plasma by affinity, anion exchange, and gel filtration chromatography. The protein gave a single band in overloaded analytical disc gel electrophoresis. The molecular weight was 54,000 and E1%/1 cm at 280 nm, corrected for thyroxine (T4) absorbance, was 6.17. Six preparations of TBG contained from 0.09 to 0.64 mol of T4/mol; the TBG used in this study contained 0.19 mol of T4 and was able to bind an additional 0.85 mol. The… CONTINUE READING

Similar Papers

Loading similar papers…